Product Description
Symmetry300 Column and Cartridge
Column Method Validation Kits
T hree columns from three different batches to test reproducibility.
Dimension Type Particle Size Part No. C18 Part No. C4
2.1 x 150 mm Column 3.5 m 186000194 186000290
4.6 x 150 mm Column 3.5 m 186000195 186000291
2.1 x 150 mm Column 5 m WAT106193 186000292
3.9 x 150 mm Column 5 m WAT106187 186000293
4.6 x 150 mm Column 5 m WAT106190 186000294
4.6 x 250 mm Column 5 m WAT106184 186000295
3.9 x 150 mm Cartridge* 5 m WAT106181
4.6 x 150 mm Cartridge* 5 m WAT106175
4.6 x 250 mm Cartridge* 5 m WAT106178
Symmetry300 Cartridge Columns
Dimension Particle Size Part No. C18
2.1 x 50 mm 3.5 m 186000199
2.1 x 100 mm 3.5 m 186000191
2.1 x 150 mm 3.5 m 186000196
4.6 x 75 mm 3.5 m 186000192
4.6 x 100 mm 3.5 m 186000193
3.9 x 150 mm 5 m WAT106169
4.6 x 150 mm 5 m WAT106163
4.6 x 250 mm 5 m WAT106160
Exceptional Separation EfficiencyDesigned with a 5 m particle size and 300 pore size, the Symmetry300 Column Cartridge offers precise separation of peptides, proteins, and small molecules. This advanced bonded silica phase optimizes resolution and reproducibility for high-quality analytical results in research and pharmaceutical environments.
Wide Chemical Compatibility and VersatilityThe cartridge withstands a broad array of organic solvents and aqueous buffers thanks to its chemically stable C18 reversed-phase material. Compatibility with a wide pH range (28) allows for varied application across complex biological and pharmaceutical samples.
Engineered for Robustness and ReusabilityCrafted from high-performance stainless steel with polymeric and silica-based packing, the cartridge delivers high mechanical strength and can be reused with proper washing protocols. Its durability allows for reliable operation under pressures up to 6000 psi and temperatures up to 60C, ensuring long service life.
FAQs of Symmetry300 Column and Cartridge :
Q: How do I use the Symmetry300 Column Cartridge in my HPLC system?
A: The Symmetry300 Column Cartridge is designed with standard HPLC end fittings, enabling compatibility with most major HPLC instruments. Simply install the cartridge in your HPLC system following the manufacturers protocol and ensure a recommended flow rate between 0.52.0 mL/min for optimal results.
Q: What applications are best suited for the Symmetry300 Column Cartridge?
A: This column excels in peptide and protein separations, analytical testing, and pharmaceutical research. Its high-resolution, C18 reversed-phase design is particularly effective for separating compounds in complex biological samples.
Q: When should I regenerate or wash the Symmetry300 Column to maintain performance?
A: Regeneration is recommended when you observe reduced separation efficiency or increased backpressure. Wash the column with suitable solvents between sample runs according to established protocols to extend cartridge lifespan and ensure reproducible outcomes.
Q: Where is the Symmetry300 Column Cartridge manufactured and supplied?
A: The Symmetry300 Column Cartridge is manufactured, exported, and supplied by reputable partners in India, serving international markets with reliable high-quality chromatography solutions.
Q: What is the benefit of the advanced bonded silica packing technology?
A: The advanced bonded silica packing in the Symmetry300 Column provides superior mechanical strength, high separation efficiency, and stable operation under various chemical conditions, making it ideal for demanding HPLC applications.
Q: How chemically compatible is the Symmetry300 Cartridge with different solvents?
A: It offers excellent compatibility with a broad range of organic solvents and aqueous buffers, accommodating most sample requirements and mobile phases used in peptide, protein, and pharmaceutical analyses.