Gel Permeation/Size Exclusion (GPC/SEC) HPLC COLUMN

9000 INR/Piece

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Product Details:

Control Type External (through HPLC system)
Shape Cylindrical
Temperature Resistance Up to 60C
Features High resolution, wide molecular weight range, low sample adsorption, compatible with most organic solvents
Accuracy 0.2% (molecular weight calibration)
Type GPC/SEC HPLC Column
Dimension (L*W*H) 300 mm 7.8 mm i.d.
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Gel Permeation/Size Exclusion (GPC/SEC) HPLC COLUMN Price And Quantity

Minimum Order Quantity 3 Piece
Price 9000 INR/Piece

Gel Permeation/Size Exclusion (GPC/SEC) HPLC COLUMN Product Specifications

Type GPC/SEC HPLC Column
Material High purity polystyrene divinylbenzene (PS-DVB)
Shape Cylindrical
Equipment Type Liquid Chromatography Column
Control Type External (through HPLC system)
Dimension (L*W*H) 300 mm 7.8 mm i.d.
Accuracy 0.2% (molecular weight calibration)
Equipment Materials Stainless Steel Column Housing
Features High resolution, wide molecular weight range, low sample adsorption, compatible with most organic solvents
Temperature Resistance Up to 60C
Application Polymer and biomolecule molecular weight analysis

Gel Permeation/Size Exclusion (GPC/SEC) HPLC COLUMN Trade Information

Supply Ability 3-4 Piece Per Day
Delivery Time 3-4 Week

Product Description

Gel Permeation/Size Exclusion (GPC/SEC) HPLC COLUMN

Gel permeation chromatography (GPC) and size exclusion chromatography (SEC) are names applied to the most popular technique for measuring the molecular weight distribution of natural and synthetic polymers, a property that affects many of the physical parameters of materials such as strength, toughness and chemical resistance.

GPC and SEC are liquid chromatographic techniques that separate individual polymer chains on the basis of their size in solution and not on their chemistry.

Gel permeation chromatography (GPC) is the name used to describe the analysis of polymers in organic solvents, such as tetrahydrofuran.

Size exclusion chromatography (SEC) is the name used to describe the analysis of polymers in water and water-based solvents, such as buffer solutions.

GPC/SEC is the only established method for obtaining a
comprehensive understanding of a polymers molecular weight distribution.

Agilent offers a comprehensive portfolio of GPC/SEC columns and calibrants for high performance separations based on molecular size in solution.

A portfolio for analysis of synthetic and natural polymers.

A wide selection of polymer standards to cover the range of applications in organic and water based solvents.

PL aquagel-OH-series, for aqueous SEC separations, and PLgel, for organic polymer applications, are available in mixed and individual pore sizes across a range of particle sizes, to cover the full spectrum of molecular weights.

Prep scale columns are available, along with narrow bore columns and columns designed for specific applications.

Reliable Molecular Weight Analysis

Utilize this GPC/SEC HPLC Column to determine molecular weights of polymers and biomolecules with exceptional precision and reproducibility. The cross-linked PS-DVB gel ensures consistent results, even with a wide range of sample types, while advanced detection compatibility (RI, UV, light scattering) expands analytical applications.

Robust Design & Broad Compatibility

Fabricated from stainless steel and packed with high-purity PS-DVB, this column offers chemical and physical resilience for prolonged laboratory use. It supports standard HPLC fittings, a wide sample pH range (213), and compatibility with most organic solvents, making integration into existing chromatography setups seamless for exporters, importers, manufacturers, and research facilities.

FAQs of Gel Permeation/Size Exclusion (GPC/SEC) HPLC COLUMN :

Q: How is this GPC/SEC HPLC column used in molecular weight analysis?

A: The column separates polymers or biomolecules according to their size using a cross-linked PS-DVB gel. The molecular weight can then be determined using detectors such as refractive index, UV, or light scattering, providing accurate characterization for research or quality control.

Q: What is the recommended process for operating the column?

A: Operate the column with a flow rate of 0.51.0 mL/min and a maximum sample load of 200 L. Use compatible organic solvents and maintain system pressures below 60 bar to ensure consistent performance and prolong column lifespan.

Q: When should the column be calibrated or maintained?

A: Calibration is recommended before initial usage and routinely as required to maintain the accuracy (0.2%) of molecular weight determination. Regular flushing and using filtered solvents help sustain column efficiency and performance.

Q: Where can this GPC/SEC HPLC column be integrated?

A: It is compatible with standard liquid chromatography systems utilizing standard HPLC fittings. The column is suitable for analytical laboratories, universities, and industrial R&D facilities across India who perform polymer and biomolecule analyses.

Q: What are the benefits of using high-purity PS-DVB as column packing material?

A: High-purity PS-DVB offers low sample adsorption, high chemical stability, and broad solvent compatibility. These features not only enable accurate sizing over a wide molecular weight range but also enhance the robustness and lifespan of the column.

Q: How does this column support various detection methods?

A: The column is optimized for use with refractive index, UV, and light scattering detectors. This versatility allows the analysis of diverse sample compositions and complex mixtures in polymer and biomolecule studies.

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