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Basal Medium Eagle (BME)

Basal Medium Eagle (BME)

Product Details:

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Product Description

Basal Medium Eagle (BME)

Description

Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. Gibco® BME has since been used for other human lines, including WI-38 and MRC-5.
This BME is modified as follows:
WithWithout
• Phenol Red• L-glutamine

• HEPES

The complete formulation is available.

Gibco® BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law

cGMP Manufacturing and Quality System
Gibco® BME is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® BME product made in our Grand Island facility (21010-046). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). BME uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5-10% CO2 environment to maintain physiological pH

 


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