Product Description
AGILENT ZORBAX Eclipse Plus HPLC COLUMN
Eclipse Plus columns provide the ultimate in performance for silica-based columns. Peak shape for the Eclipse Plus is excellent for the most challenging basic compounds, improving efficiency and resolution with these sample types. These results are achieved by improvements in the silica manufacturing and bonding technology, which is completely controlled by Agilent. Eclipse Plus columns are the ideal first choice for method development of all samples. To achieve fast method development and superior productivity, choose an Eclipse Plus column with high-resolution 1.8 µm particles. For standard methods, conventional 5 µm Eclipse Plus Columns and Rapid Resolution 3.5 µm Eclipse Plus columns are your best choices. With all particle sizes, easy method transfer is possible.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
PART NO. 820950-936 Eclipse Plus C18 Grd,4.6x12.5mm,5um,4pk PART NO. 820999-901 High Perf. ZORBAX Guard Fittings Kit Includes low-volume guard holder, inlet end fitting (2), outlet end fitting with integrated column connector, and PEEK fingertight fitting,seals (2/pk)
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Superior Peak Shape for Complex SamplesEngineered with double endcapping, the Eclipse Plus HPLC column enhances peak symmetry, minimizing tailing even for basic compounds and improving quantitation. This design promotes high resolution and consistent results, ideal for challenging sample matrices and pharmaceutical analyses.
Reliable Performance for Analytical SeparationsFeaturing a 5 m particle size and 100 pore size, this column achieves efficient separation at analytical scale. Its compatibility with pressures up to 600 bar and temperature resistance up to 60C supports demanding method requirements. The columns dimensions of 250 x 4.6 mm offer substantial sample capacity, perfectly matching standard analytical workflows.
Versatile Integration with HPLC SystemsWith standard 10-32 thread fittings and universal compatibility, the ZORBAX Eclipse Plus column integrates seamlessly into most HPLC instruments. Its leak-tight stainless steel housing and high-purity silica packing provide robustness and longevity, making it a dependable choice for continual laboratory use.
FAQs of AGILENT ZORBAX Eclipse Plus HPLC COLUMN:
Q: How does the double endcapped C18 phase of the Eclipse Plus column improve separation?
A: The double endcapped C18 phase enhances peak shape by minimizing active silanol interactions. This results in sharper, more symmetrical peaks, especially for basic and polar analytes, thereby improving accuracy and quantitation in your HPLC analyses.
Q: What are the recommended applications for the AGILENT ZORBAX Eclipse Plus HPLC Column?
A: This column is ideal for high-performance liquid chromatography applications such as pharmaceutical quality control, chemical analysis, and routine analytical separations where high resolution and reproducibility are required.
Q: When should I use a column with a 5 m particle size and 100 pore size?
A: Columns with 5 m particles and 100 pores are well-suited for general analytical HPLC methods, providing a balance between backpressure and separation efficiency, especially for small molecule analytes.
Q: Where can I install the Eclipse Plus HPLC Column in my setup?
A: The column is designed with standard 10-32 threaded fittings, enabling easy installation into most analytical HPLC instruments from various manufacturers due to its universal compatibility.
Q: What process should be followed for using this column in an HPLC system?
A: Mount the column with compatible fittings, equilibrate with the mobile phase within the 29 pH range, and maintain operation below 60C and 600 bar for optimal performance. Regular flushing ensures longevity and consistent analytical results.
Q: How does the Eclipse Plus column benefit mass spectrometry applications?
A: Its ultra-high purity silica and extra dense bonding produce very low bleed, reducing background interference and improving sensitivity in LC-MS analyses, facilitating accurate detection of trace compounds.
Q: Is the column leak-proof and suitable for high-pressure HPLC systems?
A: Yes, the stainless steel housing and precision packing deliver robust, leak-tight operation, reliably handling pressures up to 600 bar in modern analytical HPLC systems.